RNA Affinity Chromatography
نویسندگان
چکیده
منابع مشابه
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We describe a fast and simple one-step affinity-purification method for the isolation of specific RNA-binding proteins. An in vitro-transcribed hybrid RNA consisting of an aptamer sequence with high binding specificity to the antibiotic streptomycin and a putative protein-binding RNA sequence is incubated with crude extract. After complex formation, the sample is applied to an affinity column c...
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Acidic proteins with affinity to RNA from cytoplasmic fraction of Krebs II Ascites cells were isolated by means of affinity chromatography on RNA-Sepharose CN-Br-columns. Stepwise elution with 350 mM [K+] and 1000 mM [K+] removed two fractions of proteins both of which are required for the formation of 40S- or 80S-initiation complexes and for efficient translation.
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تاریخ انتشار 2017